Alkondon M, Albuquerque E X
Department of Pharmacology and Experimental Therapeutics, University of Maryland School of Medicine, Baltimore 21201.
Neurosci Lett. 1994 Aug 1;176(2):152-6. doi: 10.1016/0304-3940(94)90070-1.
The presence of a functional nicotinic acetylcholine receptor (nAChR) in rat olfactory bulb (OB) neurons was investigated using the whole-cell patch-clamp technique. Acetylcholine (ACh) or other nicotinic agonists elicited fast-decaying whole-cell currents in cultured OB neurons. ACh-evoked currents could be blocked by the highly selective competitive nicotinic antagonists alpha-bungarotoxin (alpha-BGT, 10 nM) and methyllycaconitine (MLA, 1 nM) and exhibited strong inward rectification in the presence of intracellular Mg2+. The properties of the nicotinic currents in OB neurons were similar to those of type IA currents elicited by nicotinic agonists in rat hippocampal neurons. The present results suggest that the fast-decaying nicotinic currents evoked in OB neurons are subserved by nAChRs containing alpha 7 subunits.
运用全细胞膜片钳技术，研究了大鼠嗅球（OB）神经元中功能性烟碱型乙酰胆碱受体（nAChR）的存在情况。乙酰胆碱（ACh）或其他烟碱型激动剂在培养的OB神经元中引发了快速衰减的全细胞电流。ACh诱发的电流可被高选择性竞争性烟碱型拮抗剂α-银环蛇毒素（α-BGT，10 nM）和甲基lycaconitine（MLA，1 nM）阻断，并且在细胞内Mg2+存在的情况下表现出强烈的内向整流。OB神经元中烟碱型电流的特性与大鼠海马神经元中烟碱型激动剂引发的IA型电流的特性相似。目前的结果表明，OB神经元中诱发的快速衰减烟碱型电流由含有α7亚基的nAChRs介导。
