鉴定经紫外线照射尾蚴免疫的C57B1/6小鼠脾细胞所识别的曼氏血吸虫抗原。
Identification of Schistosoma mansoni antigens recognized by spleen cells of C57B1/6 mice immunized with ultraviolet-irradiated cercariae.
作者信息
Osman A, el Ridi R, Guirguis N, Dean D A
机构信息
Zoology Department, Faculty of Science, Cairo University, Egypt.
出版信息
Int J Parasitol. 1994 Nov;24(7):943-50. doi: 10.1016/0020-7519(94)90158-9.
PMID:7883445
Abstract
Spleen cells of C57B1/6 mice immunized twice with Schistosoma mansoni cercariae attenuated by ultraviolet irradiation proliferated and produced interleukin-(II)-2 and/or II-4 in response to both soluble schistosomular and adult worm antigens of 72-68, 60-62, 50, 45, 29.5 and 28 kDa. All of these bands, except the 45 kDa, were also recognized by serum antibodies in Western blotting.
摘要
用紫外线照射减毒的曼氏血吸虫尾蚴对C57B1/6小鼠进行两次免疫后，其脾细胞在受到72 - 68、60 - 62、50、45、29.5和28 kDa的可溶性血吸虫幼虫及成虫抗原刺激时会增殖，并产生白细胞介素-2和/或白细胞介素-4。除了45 kDa的条带外，所有这些条带在蛋白质印迹法中也能被血清抗体识别。
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