Harel J, Fairbrother J, Forget C, Desautels C, Moore J
GREMIP, Faculté de Médecine Vétérinaire, Université de Montréal, St-Hyacinthe, Qué., Canada.
Vet Microbiol. 1993 Dec;38(1-2):139-55. doi: 10.1016/0378-1135(93)90081-h.
In this study, 91 F165-positive Escherichia coli isolated from calves and piglets with diarrhea or septicemia were characterized with respect to receptor binding specificity, presence of the aerobactin system, production of colicin V, resistance to the bactericidal effects of serum. Although most F165-positive isolates shared similar DNA sequences with pap operon sequences, less than half of these isolates demonstrated MRHA to P antigen of human red blood cells and Forssman antigen of sheep red blood cells recognized by P and F (or Prs) adhesins respectively. Certain F165-positive isolates sharing similar DNA sequences with both pap and sfa operon sequences demonstrated mannose-resistant hemagglutination of sheep erythrocytes, as observed in human uropathogenic E. coli possessing the prs operon. Most isolates caused mannose-resistant, neuraminidase-resistant hemagglutination of human, equine, feline, and bovine erythrocytes. Thus, F165-positive isolates express one or more adhesins with different receptor binding specificities. An association was observed between the various receptor binding specificities and serogroup. Most F165-positive isolates possessed the aerobactin system and were resistant to the bactericidal effects of serum, but only 38.5% isolates produced colicin V.
在本研究中，从患有腹泻或败血症的犊牛和仔猪中分离出91株F165阳性大肠杆菌，对其受体结合特异性、气杆菌素系统的存在、产V型大肠杆菌素情况以及对血清杀菌作用的抗性进行了表征。尽管大多数F165阳性分离株与pap操纵子序列具有相似的DNA序列，但这些分离株中不到一半表现出对人红细胞P抗原和绵羊红细胞Forssman抗原的甘露糖抗性血凝反应（MRHA），P抗原和Forssman抗原分别由P和F（或Prs）黏附素识别。某些与pap和sfa操纵子序列均具有相似DNA序列的F165阳性分离株表现出对绵羊红细胞的甘露糖抗性血凝反应，这在具有prs操纵子的人尿道致病性大肠杆菌中也有观察到。大多数分离株引起对人、马、猫和牛红细胞的甘露糖抗性、神经氨酸酶抗性血凝反应。因此，F165阳性分离株表达一种或多种具有不同受体结合特异性的黏附素。观察到各种受体结合特异性与血清群之间存在关联。大多数F165阳性分离株具有气杆菌素系统且对血清杀菌作用具有抗性，但只有38.5%的分离株产V型大肠杆菌素。
