GABA与酪氨酸羟化酶样免疫反应性在兔视网膜无长突细胞中的共定位。

Co-localization of GABA- and tyrosine hydroxylase-like immunoreactivities in amacrine cells of the rabbit retina.

作者信息

Young H M

机构信息

Department of Physiology and Pharmacology, University of Queensland, Australia.

出版信息

Vision Res. 1994 Apr;34(8):995-9. doi: 10.1016/0042-6989(94)90003-5.

DOI:10.1016/0042-6989(94)90003-5

PMID:7909184

Abstract

Previous studies have shown that many of the tyrosine hydroxylase-immunoreactive (TH-IR) amacrine cells in the retina of mammals also show GABA-like immunoreactivity (GABA-IR). However such co-localization has yet to be demonstrated in the rabbit retina. In this study the proportion of TH-IR amacrine cells that show GABA-IR has been determined in the retina of the cat, rat and rabbit following fixation with 4% paraformaldehyde plus varying concentrations of glutaraldehyde. In the retina of the cat and rat, most of the TH-IR amacrine cells showed GABA-IR following fixation using low concentrations (0.01 or 0.1%) of glutaraldehyde. However in the rabbit retina very few of the TH-IR amacrine cells showed GABA-IR unless the glutaraldehyde concentration in the fixative was 0.5% or greater. The results suggest that the dopaminergic amacrine cells in the rabbit retina contain only very low levels of GABA, which are close to the detection limit of the antibody.

摘要

先前的研究表明，哺乳动物视网膜中许多酪氨酸羟化酶免疫反应性（TH-IR）无长突细胞也显示出γ-氨基丁酸样免疫反应性（GABA-IR）。然而，这种共定位在兔视网膜中尚未得到证实。在本研究中，在用4%多聚甲醛加不同浓度戊二醛固定后，已确定猫、大鼠和兔视网膜中显示GABA-IR的TH-IR无长突细胞的比例。在猫和大鼠的视网膜中，使用低浓度（0.01%或0.1%）戊二醛固定后，大多数TH-IR无长突细胞显示出GABA-IR。然而，在兔视网膜中，除非固定剂中的戊二醛浓度为0.5%或更高，否则很少有TH-IR无长突细胞显示出GABA-IR。结果表明，兔视网膜中的多巴胺能无长突细胞仅含有非常低水平的GABA，接近抗体的检测极限。