位点特异性修饰对限制性核酸内切酶和DNA修饰甲基转移酶的影响。
Effect of site-specific modification on restriction endonucleases and DNA modification methyltransferases.
作者信息
McClelland M, Nelson M, Raschke E
机构信息
California Institute of Biological Research, La Jolla 92037.
出版信息
Nucleic Acids Res. 1994 Sep;22(17):3640-59. doi: 10.1093/nar/22.17.3640.
Abstract
Restriction endonucleases have site-specific interactions with DNA that can often be inhibited by site-specific DNA methylation and other site-specific DNA modifications. However, such inhibition cannot generally be predicted. The empirically acquired data on these effects are tabulated for over 320 restriction endonucleases. In addition, a table of known site-specific DNA modification methyltransferases and their specificities is presented along with EMBL database accession numbers for cloned genes.
摘要
限制性核酸内切酶与DNA具有位点特异性相互作用，这种相互作用常常会受到位点特异性DNA甲基化和其他位点特异性DNA修饰的抑制。然而，这种抑制作用通常无法预测。针对320多种限制性核酸内切酶，将关于这些效应的经验性获得的数据制成了表格。此外，还列出了已知的位点特异性DNA修饰甲基转移酶及其特异性的表格，以及克隆基因的EMBL数据库登录号。
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