Miller K, Reich E, Grau T, Hautmann R
Urologische Universitätsklinik, Ulm.
Urol Res. 1994;22(2):79-83. doi: 10.1007/BF00310996.
In vitro experiments were performed on human bladder carcinoma cells to evaluate the uptake of aluminum-chlorophthalocyanine (AISPc) and the subcellular target of phototoxicity. In order to quantify the correlation of intracellular uptake and incubation time and to identify the primary subcellular target of phototoxicity, fluorescence and absorption measurements have been carried out as well as electron microscopic studies. Absorption and fluorescence measurements showed the largest value after 24 h of incubation time. Fluorescence microscopic studies suggested the sensitizer to be located in a brighter patch within cytoplasm. Electron microscopic studies using DAB (3,3' diaminobenzidine) staining showed that the mitochondria are the primary target of phototoxic activity of AlSPc and that the majority of vacuoles of treated cells were originally mitochondria.
对人膀胱癌细胞进行了体外实验，以评估氯铝酞菁（AlSPc）的摄取情况及光毒性的亚细胞靶点。为了量化细胞内摄取与孵育时间的相关性，并确定光毒性的主要亚细胞靶点，进行了荧光和吸收测量以及电子显微镜研究。吸收和荧光测量显示，孵育24小时后数值最大。荧光显微镜研究表明，敏化剂位于细胞质内较亮的区域。使用DAB（3,3'-二氨基联苯胺）染色的电子显微镜研究表明，线粒体是AlSPc光毒性活性的主要靶点，且处理过的细胞中的大多数液泡原本是线粒体。
