拟南芥中编码聚腺苷酸结合蛋白的多基因家族成员PAB2的分子特征分析
Molecular characterization of PAB2, a member of the multigene family coding for poly(A)-binding proteins in Arabidopsis thaliana.
作者信息
Hilson P, Carroll K L, Masson P H
机构信息
Laboratory of Genetics, University of Wisconsin, Madison 53706.
出版信息
Plant Physiol. 1993 Oct;103(2):525-33. doi: 10.1104/pp.103.2.525.
Abstract
The poly(A) tail of eukaryotic mRNAs associates with poly(A)-binding (PAB) proteins whose role in mRNA translation and stability is being intensively investigated. Very little is known about the structure and function of the PAB genes in plants. We have cloned multiple PAB-related sequences from Arabidopsis thaliana. Results suggest that PAB proteins are encoded by a multigene family. One member of this family (PAB2) is expressed in root and shoot tissues. The complete nucleotide sequence of PAB2 was determined. Study of the predicted PAB2 protein reveals a similarity in structure among vertebrate, insect, yeast, and plant PAB proteins. All contain two highly conserved domains: an amino-terminal sequence formed by four RNA recognition motifs and an uncharacterized carboxyl-terminal region of 69 to 71 amino acids. Possible roles for the carboxyl-terminal conserved domain are discussed in view of recently published data concerning the structure and function of PAB proteins.
摘要
真核生物信使核糖核酸（mRNA）的聚腺苷酸（poly(A)）尾与聚腺苷酸结合（PAB）蛋白相关联，目前人们正在深入研究这些蛋白在mRNA翻译和稳定性中的作用。关于植物中PAB基因的结构和功能，人们所知甚少。我们从拟南芥中克隆了多个与PAB相关的序列。结果表明，PAB蛋白由一个多基因家族编码。该家族的一个成员（PAB2）在根和茎组织中表达。测定了PAB2的完整核苷酸序列。对预测的PAB2蛋白的研究揭示了脊椎动物、昆虫、酵母和植物PAB蛋白在结构上的相似性。它们都包含两个高度保守的结构域：一个由四个RNA识别基序形成的氨基末端序列和一个由69至71个氨基酸组成的未明确特征的羧基末端区域。鉴于最近发表的有关PAB蛋白结构和功能的数据，讨论了羧基末端保守结构域可能的作用。
相似文献
1
Molecular characterization of PAB2, a member of the multigene family coding for poly(A)-binding proteins in Arabidopsis thaliana.拟南芥中编码聚腺苷酸结合蛋白的多基因家族成员PAB2的分子特征分析
Plant Physiol. 1993 Oct;103(2):525-33. doi: 10.1104/pp.103.2.525.
2
Conserved expression of Arabidopsis thaliana poly (A) binding protein 2 (PAB2) in distinct vegetative and reproductive tissues.拟南芥多聚（A）结合蛋白2（PAB2）在不同营养组织和生殖组织中的保守表达。
Plant J. 2000 May;22(3):199-210. doi: 10.1046/j.1365-313x.2000.00720.x.
3
Arabidopsis thaliana poly (A) binding protein 2 (PAB2) functions in yeast translational and mRNA decay processes.拟南芥多聚（A）结合蛋白2（PAB2）在酵母翻译和mRNA降解过程中发挥作用。
Plant J. 2000 May;22(3):187-98. doi: 10.1046/j.1365-313x.2000.00721.x.
4
The wheat poly(A)-binding protein functionally complements pab1 in yeast.
Eur J Biochem. 1997 Jan 15;243(1-2):350-7. doi: 10.1111/j.1432-1033.1997.0350a.x.
5
Four distinct classes of proteins as interaction partners of the PABC domain of Arabidopsis thaliana Poly(A)-binding proteins.作为拟南芥多聚腺苷酸结合蛋白PABC结构域相互作用伙伴的四类不同蛋白质。
Mol Genet Genomics. 2005 Feb;272(6):651-65. doi: 10.1007/s00438-004-1090-9. Epub 2005 Jan 14.
6
Differential organ-specific expression of three poly(A)-binding-protein genes from Arabidopsis thaliana.拟南芥中三个聚腺苷酸结合蛋白基因在不同器官中的特异性表达
Proc Natl Acad Sci U S A. 1993 Jul 15;90(14):6686-90. doi: 10.1073/pnas.90.14.6686.
7
A novel family of plant splicing factors with a Zn knuckle motif: examination of RNA binding and splicing activities.具有锌指模体的新型植物剪接因子家族：RNA结合和剪接活性检测
Plant Mol Biol. 1999 Mar;39(4):761-73. doi: 10.1023/a:1006129615846.
8
An Arabidopsis serine/threonine kinase homologue with an epidermal growth factor repeat selected in yeast for its specificity for a thylakoid membrane protein.一种拟南芥丝氨酸/苏氨酸激酶同源物，其具有表皮生长因子重复序列，在酵母中因其对类囊体膜蛋白的特异性而被筛选出来。
Proc Natl Acad Sci U S A. 1992 Nov 15;89(22):10989-92. doi: 10.1073/pnas.89.22.10989.
9
Sequencing and characterization of the kinesin-related genes katB and katC of Arabidopsis thaliana.拟南芥驱动蛋白相关基因katB和katC的测序与特征分析
Plant Mol Biol. 1994 Aug;25(5):865-76. doi: 10.1007/BF00028881.
10
Novel Drosophila melanogaster genes encoding RRM-type RNA-binding proteins identified by a degenerate PCR strategy.通过简并PCR策略鉴定的编码RRM型RNA结合蛋白的新型黑腹果蝇基因。
Gene. 1995 Mar 10;154(2):187-92. doi: 10.1016/0378-1119(94)00840-o.
引用本文的文献
1
Toward a systems view on RNA-binding proteins and associated RNAs in plants: Guilt by association.从系统角度看待植物中的 RNA 结合蛋白和相关 RNA：关联的罪责。
Plant Cell. 2023 May 29;35(6):1708-1726. doi: 10.1093/plcell/koac345.
2
Comparative proteomics of the two T. brucei PABPs suggests that PABP2 controls bulk mRNA.两种 T. brucei PABPs 的比较蛋白质组学研究表明，PABP2 控制大量 mRNA。
PLoS Negl Trop Dis. 2018 Jul 24;12(7):e0006679. doi: 10.1371/journal.pntd.0006679. eCollection 2018 Jul.
3
Plant growth and fertility requires functional interactions between specific PABP and eIF4G gene family members.植物生长和繁殖需要特定的聚腺苷酸结合蛋白（PABP）和真核起始因子4G（eIF4G）基因家族成员之间的功能相互作用。
PLoS One. 2018 Jan 30;13(1):e0191474. doi: 10.1371/journal.pone.0191474. eCollection 2018.
4
Class II members of the poly(A) binding protein family exhibit distinct functions during Arabidopsis growth and development.聚腺苷酸结合蛋白家族的II类成员在拟南芥生长发育过程中表现出不同的功能。
Translation (Austin). 2017 Feb 17;5(1):e1295129. doi: 10.1080/21690731.2017.1295129. eCollection 2017.
5
Phylogenetic analysis reveals dynamic evolution of the poly(A)-binding protein gene family in plants.系统发育分析揭示了植物中多聚腺苷酸结合蛋白基因家族的动态进化。
BMC Evol Biol. 2014 Nov 25;14:238. doi: 10.1186/s12862-014-0238-4.
6
Differential localization of the two T. brucei poly(A) binding proteins to the nucleus and RNP granules suggests binding to distinct mRNA pools.两种 T. brucei 多聚（A）结合蛋白在核和 RNP 颗粒中的差异定位表明它们与不同的 mRNA 池结合。
PLoS One. 2013;8(1):e54004. doi: 10.1371/journal.pone.0054004. Epub 2013 Jan 30.
7
Functional analysis of the Arabidopsis thaliana poly(A) binding protein PAB5 gene promoter in Nicotiana tabacum.拟南芥多聚腺苷酸结合蛋白PAB5基因启动子在烟草中的功能分析
Plant Cell Rep. 2008 Dec;27(12):1811-9. doi: 10.1007/s00299-008-0597-7. Epub 2008 Sep 9.
8
Nitrogen-dependent posttranscriptional regulation of the ammonium transporter AtAMT1;1.铵转运蛋白AtAMT1;1的氮依赖性转录后调控
Plant Physiol. 2007 Feb;143(2):732-44. doi: 10.1104/pp.106.093237. Epub 2006 Dec 15.
9
Four distinct classes of proteins as interaction partners of the PABC domain of Arabidopsis thaliana Poly(A)-binding proteins.作为拟南芥多聚腺苷酸结合蛋白PABC结构域相互作用伙伴的四类不同蛋白质。
Mol Genet Genomics. 2005 Feb;272(6):651-65. doi: 10.1007/s00438-004-1090-9. Epub 2005 Jan 14.
10
Identification and characterization of proteins that interact with the carboxy terminus of poly(A)-binding protein and inhibit translation in vitro.与聚腺苷酸结合蛋白羧基末端相互作用并在体外抑制翻译的蛋白质的鉴定与表征。
Plant Mol Biol. 2004 Jan;54(1):85-98. doi: 10.1023/B:PLAN.0000028771.70969.6b.
本文引用的文献
1
Selection of AUG initiation codons differs in plants and animals.植物和动物中AUG起始密码子的选择有所不同。
EMBO J. 1987 Jan;6(1):43-8. doi: 10.1002/j.1460-2075.1987.tb04716.x.
2
mRNA polyadenylate-binding protein: gene isolation and sequencing and identification of a ribonucleoprotein consensus sequence.信使核糖核酸聚腺苷酸结合蛋白：基因分离、测序及核糖核蛋白共有序列的鉴定
Mol Cell Biol. 1986 Aug;6(8):2932-43. doi: 10.1128/mcb.6.8.2932-2943.1986.
3
A single gene from yeast for both nuclear and cytoplasmic polyadenylate-binding proteins: domain structure and expression.酵母中一个编码核和细胞质聚腺苷酸结合蛋白的单一基因：结构域结构与表达
Cell. 1986 Jun 20;45(6):827-35. doi: 10.1016/0092-8674(86)90557-x.
4
A single domain of yeast poly(A)-binding protein is necessary and sufficient for RNA binding and cell viability.酵母聚腺苷酸结合蛋白的单个结构域对于RNA结合和细胞活力而言是必要且充分的。
Mol Cell Biol. 1987 Sep;7(9):3268-76. doi: 10.1128/mcb.7.9.3268-3276.1987.
5
Improved M13 phage cloning vectors and host strains: nucleotide sequences of the M13mp18 and pUC19 vectors.改良的M13噬菌体克隆载体和宿主菌株：M13mp18和pUC19载体的核苷酸序列。
Gene. 1985;33(1):103-19. doi: 10.1016/0378-1119(85)90120-9.
6
Human mRNA polyadenylate binding protein: evolutionary conservation of a nucleic acid binding motif.人类mRNA聚腺苷酸结合蛋白：核酸结合基序的进化保守性
Nucleic Acids Res. 1987 Jun 25;15(12):4771-87. doi: 10.1093/nar/15.12.4771.
7
Expression of the poly(A)-binding protein during development of Xenopus laevis.非洲爪蟾发育过程中多聚腺苷酸结合蛋白的表达
Mol Cell Biol. 1989 Jun;9(6):2756-60. doi: 10.1128/mcb.9.6.2756-2760.1989.
8
The poly(A) binding protein is required for poly(A) shortening and 60S ribosomal subunit-dependent translation initiation.聚腺苷酸结合蛋白是聚腺苷酸缩短和60S核糖体亚基依赖性翻译起始所必需的。
Cell. 1989 Sep 8;58(5):857-67. doi: 10.1016/0092-8674(89)90938-0.
9
The poly(A)-poly(A)-binding protein complex is a major determinant of mRNA stability in vitro.聚腺苷酸-聚腺苷酸结合蛋白复合体是体外mRNA稳定性的主要决定因素。
Mol Cell Biol. 1989 Feb;9(2):659-70. doi: 10.1128/mcb.9.2.659-670.1989.
10
Essential large transcripts of the maize Spm transposable element are generated by alternative splicing.玉米Spm转座元件的必需大转录本是通过可变剪接产生的。
Cell. 1989 Aug 25;58(4):755-65. doi: 10.1016/0092-8674(89)90109-8.
Zotero 插件