鼠伤寒沙门氏菌孔蛋白的生物学活性——致死性、施瓦茨曼反应和致热原性。

Biological activities--lethality, Shwartzman reaction and pyrogenicity--of Salmonella typhimurium porins.

作者信息

Galdiero F, Sommese L, Scarfogliero P, Galdiero M

机构信息

Istituto di Microbiologia, Facoltà di Medicina e Chirurgia, Seconda Università degli Studi di Napoli, Italy.

出版信息

Microb Pathog. 1994 Feb;16(2):111-9. doi: 10.1006/mpat.1994.1012.

DOI:10.1006/mpat.1994.1012

PMID:8046999

Abstract

Porins isolated from Salmonella typhimurium and found to contain less than 0.1% w/w of LPS, were found to be lethal at a dose of 100 ng to both LPS-responder (BALB/cByJ) and non-responder (C3H/HeJ) mice sensitized with D-galactosamine. This lethal action could be prevented by anti-TNF-alpha serum given intravenously 10 min before the porin injection but not by polymyxin-B mixed with the porins in a ratio of approximately 300 moles polymyxin-B per mole of porin. The porin preparation was also pyrogenic to rabbits at a dose of 1 microgram/kg and elicited a local Shwartzman reaction when used as the sensitizing and eliciting agent; these reactions were also present when the porins were mixed with polymyxin-B.

摘要

从鼠伤寒沙门氏菌中分离出的孔蛋白，其脂多糖含量低于0.1%（重量/重量），结果发现，对于用D - 半乳糖胺致敏的脂多糖反应者（BALB/cByJ）和无反应者（C3H/HeJ）小鼠，剂量为100纳克时具有致死性。在注射孔蛋白前10分钟静脉注射抗TNF - α血清可预防这种致死作用，但以每摩尔孔蛋白约300摩尔多粘菌素 - B的比例与孔蛋白混合的多粘菌素 - B则不能预防。该孔蛋白制剂对兔子的致热剂量为1微克/千克，用作致敏剂和激发剂时会引发局部施瓦茨曼反应；当孔蛋白与多粘菌素 - B混合时也会出现这些反应。