评估三种血清学检测方法在侵袭性念珠菌病诊断中检测抗念珠菌抗体的效果。

Evaluation of three serological tests for detection of anti-candidal antibodies in diagnosis of invasive candidiasis.

作者信息

Chakrabarti A, Roy P, Kumar D, Sharma B K, Chugh K S, Panigrahi D

机构信息

Department of Medical Microbiology, Postgraduate Institute of Medical Education and Research, Chandigarh, India.

出版信息

Mycopathologia. 1994 Apr;126(1):3-7. doi: 10.1007/BF01371166.

DOI:10.1007/BF01371166

PMID:8052291

Abstract

Immunoblot detection of antibody against 47 KD cytoplasmic antigen of Candida albicans was evaluated in diagnosis of invasive candidiasis and compared to whole cell agglutination and gel diffusion tests for detection of anticandidal antibody in 64 patients. The patients included 17 with culture proved candidemia, 34 with significant candiduria (more than 10,000 colony forming units per ml of urine) and 13 with nonsignificant candiduria. Antibody against 47 KD antigen was found to be the best indicator for diagnosis of invasive candidiasis even in patients with malignancy. The sensitivity of this procedure was 82.4%, specificity 86.7%, positive predictive value 77.8%, negative predictive value 89.7% and efficacy 85.1%. The gel diffusion procedure lacked in sensitivity whereas whole cell agglutination lacked in specificity. Detection of antibody against 47 KD antigen proved to be a valuable adjunct in the diagnosis of invasive candidiasis.

摘要

对64例患者进行了免疫印迹法检测抗白色念珠菌47KD细胞质抗原抗体，以评估其在侵袭性念珠菌病诊断中的价值，并与全细胞凝集试验和凝胶扩散试验检测抗念珠菌抗体的结果进行比较。患者包括17例血培养证实为念珠菌血症、34例念珠菌尿显著（每毫升尿液中菌落形成单位超过10000个）和13例念珠菌尿不显著者。抗47KD抗原抗体被发现是侵袭性念珠菌病诊断的最佳指标，即使在恶性肿瘤患者中也是如此。该方法的敏感性为82.4%，特异性为86.7%，阳性预测值为77.8%，阴性预测值为89.7%，效能为85.1%。凝胶扩散试验缺乏敏感性，而全细胞凝集试验缺乏特异性。检测抗47KD抗原抗体被证明是侵袭性念珠菌病诊断中有价值的辅助手段。