嗜肺军团菌穿梭诱变：鉴定与宿主细胞细胞病变效应相关的基因
Shuttle mutagenesis of Legionella pneumophila: identification of a gene associated with host cell cytopathicity.
作者信息
Arroyo J, Hurley M C, Wolf M, McClain M S, Eisenstein B I, Engleberg N C
机构信息
Department of Microbiology, University of Michigan Medical School, Ann Arbor 48109.
出版信息
Infect Immun. 1994 Sep;62(9):4075-80. doi: 10.1128/iai.62.9.4075-4080.1994.
Abstract
We performed shuttle mutagenesis of Legionella pneumophila. Mutants were screened for reduced cellular infectivity. Approximately 10% of the mutants had decreased cytopathicity. The DNA sequence of one locus was determined; the inferred amino acid sequence revealed homology with transport proteins including Escherichia coli TolC, Bordetella pertussis CyaE, and Alcaligenes eutrophus CzcC and CnrC.
摘要
我们对嗜肺军团菌进行了穿梭诱变。筛选出细胞感染性降低的突变体。大约10%的突变体细胞病变效应减弱。确定了一个位点的DNA序列；推导的氨基酸序列显示与包括大肠杆菌TolC、百日咳博德特氏菌CyaE以及嗜碱产碱杆菌CzcC和CnrC在内的转运蛋白具有同源性。
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