Blanchflower W J, Cannavan A, Kennedy D G
Department of Agriculture, Veterinary Sciences Division, Stormont, Belfast, UK.
Analyst. 1994 Jun;119(6):1325-8. doi: 10.1039/an9941901325.
A method has been developed for the determination of the anthelmintics fenbendazole and oxfendazole in liver and muscle samples using liquid chromatography-thermospray mass spectrometry (LC-MS). The samples were homogenized with water, sonicated with methanol and centrifuged. The supernatants were washed with light petroleum and extracted with diethyl ether-ethyl acetate. The extracts were evaporated to dryness, dissolved in mobile phase and injected into the LC-MS system. Fenbendazole and oxfendazole were measured separately using two different mobile phases. Single-ion monitoring of the positive ion at m/z 300 was used for fenbendazole and of m/z 316 for oxfendazole. The detection limits for the assay were 0.05 microgram g-1 for fenbendazole and 0.1 microgram g-1 for oxfendazole. The mean recoveries were 91% for fenbendazole and 86% for oxfendazole. The assay has been used for statutory testing purposes and for measuring the levels of fenbendazole and oxfendazole in liver and muscle from sheep after dosing with a commercial anthelmintic containing fenbendazole.
已开发出一种使用液相色谱 - 热喷雾质谱法（LC-MS）测定肝脏和肌肉样本中抗蠕虫药芬苯达唑和奥芬达唑的方法。将样本与水匀浆，用甲醇超声处理并离心。上清液用石油醚洗涤，并用乙醚 - 乙酸乙酯萃取。提取物蒸发至干，溶解于流动相后注入LC-MS系统。芬苯达唑和奥芬达唑分别使用两种不同的流动相进行测定。芬苯达唑采用m/z 300的正离子单离子监测，奥芬达唑采用m/z 316的正离子单离子监测。该测定方法的检测限为：芬苯达唑0.05微克 g⁻¹，奥芬达唑0.1微克 g⁻¹。芬苯达唑的平均回收率为91%，奥芬达唑为86%。该测定方法已用于法定检测目的，以及测量给绵羊投喂含芬苯达唑的商用抗蠕虫药后，绵羊肝脏和肌肉中芬苯达唑和奥芬达唑的含量。
