一种表征线粒体核糖核酸的简单方法。
A simple method of characterizing mitochondrial ribonucleic acid.
作者信息
Haldar D, Waters B K, Freeman D B
出版信息
Biochem J. 1975 Jun;147(3):621-3. doi: 10.1042/bj1470621.
Abstract
A simple method of isolating and characterizing RNA from L-cell mitochondria is described. The mitochondrial fraction is lysed by sodium dodecyl sulphate, and the RNA fractionated by sucrose-density-gradient centrifugation. The efficacy of proteinase K in preventing ribonuclease activity is also demonstrated.
摘要
本文描述了一种从L细胞线粒体中分离和鉴定RNA的简单方法。线粒体部分用十二烷基硫酸钠裂解，RNA通过蔗糖密度梯度离心进行分级分离。还证明了蛋白酶K在防止核糖核酸酶活性方面的功效。
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