Ockleford C D
J Microsc. 1975 Nov;105(2):193-203. doi: 10.1111/j.1365-2818.1975.tb04050.x.
A marine unicellular organism, human trophoblast tissue and cultured trophoblast cells of human origin have been satisfactorily preserved for electron microscopy without resort to washing either before dehydration or between different stages of fixation. The time required to fix and dehydrate a specimen using this method is 55 min.
一种海洋单细胞生物、人滋养层组织以及源自人的培养滋养层细胞,无需在脱水前或不同固定阶段之间进行冲洗,就能令人满意地保存用于电子显微镜观察。使用该方法固定和脱水一个标本所需的时间为55分钟。