构建一个跨越5号染色体q34 - 35区域至少7兆碱基对的酵母人工染色体（YAC）重叠群和序列标签位点（STS）图谱。

Construction of a YAC contig and a STS map spanning at least seven megabasepairs in chromosome 5q34-35.

作者信息

Lu-Kuo J M, Le Paslier D, Weissenbach J, Chumakov I, Cohen D, Ward D C

机构信息

Department of Molecular Biophysics, Yale University School of Medicine, New Haven, CT 06510.

出版信息

Hum Mol Genet. 1994 Jan;3(1):99-106. doi: 10.1093/hmg/3.1.99.

DOI:10.1093/hmg/3.1.99

PMID:8162060

Abstract

We have constructed a YAC contig containing 54 clones and a minimum of 7 Mbp of human DNA, that maps to bands q34-35 on chromosome 5. The contig was nucleated using FISH mapped cosmid clones shown to flank the t(2;5)(p23;q35) translocation breakpoint in a CD30-positive large cell lymphoma cell line. Thirty of the 54 YAC clones are non-chimeric and six span the translocation breakpoint, as determined by FISH analysis. A total of 28 YAC clone end fragments, 14 non-polymorphic YAC end STS probes and 13 polymorphic microsatellite STS markers have been used to order clones within the contig. The most distal genetic markers (D5S498 and D5S619) are separated by 15 cM based on multipoint linkage analysis. This map of overlapping clones and the set of densely spaced physical markers will promote our understanding of the 5q34-35 region and its associated genes.

摘要

我们构建了一个酵母人工染色体（YAC）重叠群，其中包含54个克隆以及至少7兆碱基对（Mbp）的人类DNA，该重叠群定位于5号染色体的q34 - 35带。该重叠群以荧光原位杂交（FISH）定位的黏粒克隆为核心构建，这些黏粒克隆在一个CD30阳性大细胞淋巴瘤细胞系中位于t(2;5)(p23;q35)易位断点两侧。通过FISH分析确定，54个YAC克隆中有30个是非嵌合的，6个跨越易位断点。总共28个YAC克隆末端片段、14个非多态性YAC末端序列标签位点（STS）探针和13个多态性微卫星STS标记已用于在重叠群内对克隆进行排序。基于多点连锁分析，最远端的遗传标记（D5S498和D5S619）相距15厘摩（cM）。这张重叠克隆图谱以及密集分布的物理标记集将促进我们对5q34 - 35区域及其相关基因的理解。