腹膜间皮细胞分泌CA 125
CA 125 secretion by peritoneal mesothelial cells.
作者信息
Zeillemaker A M, Verbrugh H A, Hoynck van Papendrecht A A, Leguit P
机构信息
Department of Surgery, Diakonessen Hospital, Utrecht, The Netherlands.
出版信息
J Clin Pathol. 1994 Mar;47(3):263-5. doi: 10.1136/jcp.47.3.263.
AIMS
To investigate the secretion of the tumour marker CA 125 by cultured human mesothelial cells; to determine if secretion of CA 125 could be observed by activating the mesothelial monolayers with different cytokines.
METHODS
Mesothelial cells were isolated from human omentum and cultured to confluent monolayers on perforated polycarbonate membranes (pore size 0.4 micron). The mesothelial monolayers were activated and the apical and basolateral secretion of CA 125 compared. To investigate the influence of cytokines, mesothelial cells were cultured and activated with recombinant interleukin-1 beta (rIL-1 beta), tumour necrosis factor-alpha (TNF-alpha) or lipopolysaccharide from Escherichia coli. The secretion of CA 125 was tested using a microparticle enzyme immunoassay.
RESULTS
Mesothelial monolayers secreted CA 125 in a polarised manner with preference for the apical side. Apical polarisation occurred irrespective of the side of the inducing stimulus (p < or = 0.05). Non-activated cultured mesothelial monolayers secreted significant quantities of CA 125, indicating constitutive production of this protein. However, CA 125 production was significantly enhanced if mesothelial cells were incubated with rIL-1 beta (p < or = 0.05), TNF-alpha (p < or = 0.05), and E coli LPS (p < or = 0.01).
CONCLUSIONS
Human mesothelial monolayers secrete CA 125 preferentially from their apical surfaces. The secretion of CA 125 can be enhanced by the inflammatory cytokines Il-1 beta, TNF-alpha, and by E coli LPS.
目的
研究培养的人腹膜间皮细胞分泌肿瘤标志物CA 125的情况；确定通过用不同细胞因子激活间皮单层是否能观察到CA 125的分泌。
方法
从人网膜分离腹膜间皮细胞，并在孔径为0.4微米的多孔聚碳酸酯膜上培养至汇合单层。激活间皮单层，比较CA 125在顶端和基底外侧的分泌情况。为研究细胞因子的影响，用重组白细胞介素-1β（rIL-1β）、肿瘤坏死因子-α（TNF-α）或大肠杆菌脂多糖培养并激活腹膜间皮细胞。使用微粒酶免疫测定法检测CA 125的分泌。
结果
间皮单层以极化方式分泌CA 125，优先分泌至顶端侧。无论诱导刺激的一侧如何，均会发生顶端极化（p≤0.05）。未激活的培养间皮单层分泌大量CA 125，表明该蛋白的组成性产生。然而，如果将腹膜间皮细胞与rIL-1β（p≤0.05）、TNF-α（p≤0.05）和大肠杆菌脂多糖（p≤0.01）孵育，CA 125的产生会显著增强。
结论
人腹膜间皮单层优先从其顶端表面分泌CA 125。炎症细胞因子IL-1β、TNF-α以及大肠杆菌脂多糖可增强CA 125的分泌。
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