[A general method for the determination of the conformation of nucleotides bound to an enzyme site. Interaction between glycogen phsophorylase b and nucleoside-5'-monophosphates].

The conformation of 5' nucleotides in the active site of glycogen phosphorylase b has been deduced from T2 relaxation time of protons H1, H8 and H2. It is shown by deuterium substitution of the purine ring in position 8 that the orientation of the base is anti in the case of strong activators like adenosine 5'-monophosphate, and syn in that of weak activators like inosine 5'-monophosphate.