在电鳐钠通道制剂中检测180 kDa蛋白质。

Detection of 180 kDa proteins in electroplax sodium channel preparations.

Ivey S, Thornhill W B, Levinson S R

Department of Biological Sciences, Delaware State University, Dover 19901.

Comp Biochem Physiol B. 1993 Nov;106(3):551-6. doi: 10.1016/0305-0491(93)90131-n.

Abstract

Co-isolating proteins (M(r) 170,000-220,000) from sodium channel preparations made from the electric organ of the electric eel (Electrophorus electricus) were detected on Western blots using monoclonal antibodies. 2. Similar protein patterns were seen on immunoblots containing immunoprecipitated protein from eel muscle and brain tissues but not heart. 3. These co-isolating proteins could be separated from the mature TTX-sensitive channel protein (M(r) 280,000) using a lentil lectin-Sepharose column. 4. The 180 kDa proteins do not appear to be channel-related and can be detected as contaminants in electroplax sodium channel preparations using the monoclonal antibodies described here.

摘要

使用单克隆抗体在蛋白质免疫印迹法中检测到，从电鳗（电鲇属电鳗）电器官制备的钠通道制剂中存在共分离蛋白（分子量170,000 - 220,000）。2. 在含有从鳗鱼肌肉和脑组织而非心脏免疫沉淀蛋白的免疫印迹上观察到相似的蛋白模式。3. 这些共分离蛋白可以使用扁豆凝集素 - 琼脂糖柱从成熟的对河豚毒素敏感的通道蛋白（分子量280,000）中分离出来。4. 180 kDa的蛋白似乎与通道无关，使用此处所述的单克隆抗体可将其检测为电板钠通道制剂中的污染物。