利用识别一种27千道尔顿抗原的单克隆抗体区分小鼠强毒株和无毒株刚地弓形虫
Differentiation between mouse-virulent and -avirulent strains of Toxoplasma gondii by a monoclonal antibody recognizing a 27-kilodalton antigen.
作者信息
Bohne W, Gross U, Heesemann J
机构信息
Institute of Hygiene and Microbiology, University of Würzburg, Germany.
出版信息
J Clin Microbiol. 1993 Jun;31(6):1641-3. doi: 10.1128/jcm.31.6.1641-1643.1993.
Using a murine monoclonal antibody, we were able to differentiate between mouse-virulent and -avirulent strains of Toxoplasma gondii. Monoclonal antibody TB6G5 was reactive with eight clinical mouse-avirulent isolates but not with mouse-virulent laboratory strains RH and BK. The antibody-reactive antigen was identified by indirect immunofluorescence and immunoblot as a 27-kDa cytoplasmic protein expressed by tachyzoites as well as by bradyzoites.
利用一种鼠单克隆抗体，我们能够区分刚地弓形虫的小鼠毒力株和无毒力株。单克隆抗体TB6G5与8株临床小鼠无毒力分离株有反应，但与小鼠毒力实验室株RH和BK无反应。通过间接免疫荧光和免疫印迹鉴定出抗体反应性抗原是速殖子和缓殖子表达的一种27 kDa的胞质蛋白。
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