Membrane properties and dendritic arborization of the intermediolateral nucleus neurons in the guinea-pig thoracic spinal cord in vitro.

The morphological and electrophysiological properties of neurons in the intermediolateral nucleus (IML) were studied in the transverse and longitudinal slice of guinea-pig thoracic spinal cord (T2-T3) using intracellular staining and recording techniques. Two morophologically different types of neurons were observed: fusiform cells with craniocaudally oriented dendrites, and multipolar cells with dendrites diffusely extending in the IML. The ratio of fusiform to multipolar cells was 4:1. The fusiform cells were identified as sympathetic preganglionic neurons (SPNs) by their antidromic responses to stimulation of the ventral root exit zone, while the multipolar cells were not antidromically activated by stimulation of this site. Both cell types showed similar resting membrane potential and input resistance. The tonic responses of these neurons to hyperpolarizing current pulses were characteristically different: the SPNs had a marked hyperpolarizing sag at the break of the pulse, caused by an A current, while the unidentified neurons showed no A current. In addition, the SPNs had much longer duration of spike and afterhyperpolarization, as well as lower frequency of spontaneous or current-evoked firing, than the unidentified neurons. These observations suggest that, in the absence of the criterion of antidromic activation by stimulation of the axon, it is still possible to differentiate SPNs from other IML neurons on the basis of morphological and electrophysiological properties of the neuron.