[赛庚啶对犬心肌肌浆网Ca2+、Mg(2+)-ATP酶活性及45Ca2+摄取功能的影响]
[Effect of cyproheptadine on Ca2+,Mg(2+)-ATPase activities and 45Ca2+ uptake function in canine myocardial sarcoplasmic reticulum].
作者信息
Xin H B, Zhang B H
机构信息
Department of Pharmacology, Beijing Medical University.
出版信息
Yao Xue Xue Bao. 1993;28(2):92-6.
The effects of cyproheptadine on Ca2+,Mg(2+)-ATPase activities and 45Ca2+ uptake function in isolated canine myocardial sarcoplasmic reticulum were studied. Cyproheptadine at concentrations of 8 x 10(-6) mol/L-2 x 10(-4) mol/L showed hardly any influence on Ca2+,Mg(2+)-ATPase activities of the myocardial sarcoplasmic reticulum in canine, but inhibited the enzyme activities (rate of inhibition: 39.85%, P < 0.01) at the concentration of 10(-3) mol/L. The myocardial sarcoplasmic reticulum was shown to take up 45Ca2+ significantly in a time-dependent manner and the 45Ca2+ uptake was 312.79 +/- 22.25 nmol/mg protein at 30 min. Cyproheptadine was found to inhibit the Ca2+ uptake of sarcoplasmic reticulum with an IC50 of 1.94 x 10(-4) mol/L.
研究了赛庚啶对离体犬心肌肌浆网中Ca2 +、Mg(2 +)-ATP酶活性及45Ca2 +摄取功能的影响。浓度为8×10(-6)mol/L - 2×10(-4)mol/L的赛庚啶对犬心肌肌浆网的Ca2 +、Mg(2 +)-ATP酶活性几乎没有任何影响，但在浓度为10(-3)mol/L时可抑制该酶活性（抑制率：39.85%，P < 0.01）。结果表明，心肌肌浆网能以时间依赖性方式显著摄取45Ca2 +，30分钟时45Ca2 +摄取量为312.79±22.25 nmol/mg蛋白。发现赛庚啶抑制肌浆网对Ca2 +的摄取，半数抑制浓度（IC50）为1.94×10(-4)mol/L。
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