子宫内膜异位症女性腹膜液的免疫抑制活性。

Immunosuppressive activity of peritoneal fluid in women with endometriosis.

作者信息

Oosterlynck D J, Meuleman C, Waer M, Koninckx P R, Vandeputte M

机构信息

Rega Institute for Medical Research, University of Leuven, Belgium.

出版信息

Obstet Gynecol. 1993 Aug;82(2):206-12.

PMID:8336865

Abstract

OBJECTIVE

To investigate the immunosuppressive effect of peritoneal fluid and follicular fluid on both natural killer-mediated cytotoxicity and phytohemagglutinin-induced lymphocyte proliferation.

METHODS

The peritoneal fluid of women with endometriosis was compared to both fertile and infertile control fluids. Lymphocytes were pretreated for 2 or 20 hours with peritoneal or follicular fluids, and their cytotoxicity toward K562 tumor cells was measured. We also investigated the phytohemagglutinin-induced stimulation of lymphocytes cocultured with peritoneal or follicular fluid.

RESULTS

Peritoneal fluid from women with endometriosis had a significantly greater immunosuppressive effect on natural killer-mediated cytotoxicity and on phytohemagglutinin stimulation of lymphocytes compared to peritoneal fluid of fertile women without endometriosis (P < .01 and P < .05, respectively). Using the peritoneal fluid of infertile women without endometriosis, these differences were significant only when compared to women with severe endometriosis. Inhibition of the natural killer activity increased when the incubation period was prolonged from 2 to 20 hours (P < .04). There was no correlation between the immunosuppressive effect of peritoneal fluid and the volume of peritoneal fluid, the day of the menstrual period, or estradiol, progesterone, prostaglandin E2, or prostaglandin F2 alpha levels. In peritoneal fluid, the factor responsible for inhibition of natural killer activity was not removed with charcoal treatment. In follicular fluid, on the other hand, the inhibition of natural killer activity decreased significantly after treatment with charcoal.

CONCLUSION

Natural killer activity is suppressed by the peritoneal fluid of women with severe endometriosis; this may be important in the pathogenesis of endometriosis. The factor responsible for the inhibition of natural killer activity in peritoneal fluid is different from that in follicular fluid.

摘要

目的

研究腹水和卵泡液对自然杀伤细胞介导的细胞毒性及植物血凝素诱导的淋巴细胞增殖的免疫抑制作用。

方法

将子宫内膜异位症患者的腹水与正常生育妇女及不孕妇女的对照腹水进行比较。淋巴细胞用腹水或卵泡液预处理2或20小时，然后检测其对K562肿瘤细胞的细胞毒性。我们还研究了与腹水或卵泡液共培养的淋巴细胞受植物血凝素诱导的刺激情况。

结果

与无子宫内膜异位症的正常生育妇女的腹水相比，子宫内膜异位症患者的腹水对自然杀伤细胞介导的细胞毒性及淋巴细胞受植物血凝素刺激的抑制作用明显更强（分别为P <.01和P <.05）。以无子宫内膜异位症的不孕妇女的腹水作为对照，这些差异仅在与重度子宫内膜异位症患者相比时才具有统计学意义。当孵育时间从2小时延长至20小时时，自然杀伤细胞活性的抑制作用增强（P <.04）。腹水的免疫抑制作用与腹水量、月经周期天数、雌二醇、孕酮、前列腺素E2或前列腺素F2α水平之间无相关性。在腹水中，用活性炭处理不能去除抑制自然杀伤细胞活性的因子。另一方面，在卵泡液中，用活性炭处理后自然杀伤细胞活性的抑制作用明显降低。