Dekker P J, Keil P, Rassow J, Maarse A C, Pfanner N, Meijer M
Institute for Molecular Cell Biology, BioCentrum Amsterdam, The Netherlands.
FEBS Lett. 1993 Sep 6;330(1):66-70. doi: 10.1016/0014-5793(93)80921-g.
A screening for yeast mutants impaired in mitochondrial protein import led to the identification of two genes (MPII and MPI2) encoding the essential components MIM44 and MIM17 of the inner membrane import machinery. We analyzed twelve additional mutants obtained in the screening and found two further complementation groups. One group represents mutants of SSC1, the gene encoding mitochondrial hsp70, an essential matrix protein required for protein import across the inner membrane. The second complementation group represents mutants of a new gene (MP13) encoding a 23 kDa integral inner membrane protein (MIM23). MIM23 is synthesized without a presequence, and its import to the inner membrane requires a membrane potential. MIM23 contains a domain homologous to half of MIM17. We speculate that MIM23 is a new member of the protein import machinery of the mitochondrial inner membrane.
一项针对线粒体蛋白导入受损的酵母突变体的筛选，导致鉴定出两个基因（MPII和MPI2），它们编码内膜导入机制的必需组分MIM44和MIM17。我们分析了在筛选中获得的另外12个突变体，发现了另外两个互补群。一组代表SSC1的突变体，SSC1是编码线粒体hsp70的基因，hsp70是内膜蛋白导入所需的一种必需的基质蛋白。第二个互补群代表一个新基因（MP13）的突变体，该基因编码一种23 kDa的内膜整合蛋白（MIM23）。MIM23在合成时没有前导序列，其导入内膜需要膜电位。MIM23包含一个与MIM17一半同源的结构域。我们推测MIM23是线粒体内膜蛋白导入机制的一个新成员。
