Galleni M, Lakaye B, Lepage S, Jamin M, Thamm I, Joris B, Frère J M
Laboratoire d'Enzymologie, Université de Liège, Sart-Tilman, Belgium.
Biochem J. 1993 Apr 1;291 ( Pt 1)(Pt 1):19-21. doi: 10.1042/bj2910019.
A new method for the identification and quantification of penicillin-binding proteins is described which uses fluorescein-coupled penicillins. It allows the rapid detection of 0.2 pmol with the naked eye and 2 fmol with the help of an A.L.F. automatic DNA sequencer. Direct labelling can also be performed on whole bacterial cells.
本文描述了一种鉴定和定量青霉素结合蛋白的新方法，该方法使用荧光素偶联青霉素。它能够用肉眼快速检测出0.2皮摩尔，借助A.L.F.自动DNA测序仪可检测出2飞摩尔。直接标记也可在完整细菌细胞上进行。
Zotero 插件