Tieben L M, ter Schegget J, Minnaar R P, Bouwes Bavinck J N, Berkhout R J, Vermeer B J, Jebbink M F, Smits H L
Department of Dermatology, University Hospital Leiden, The Netherlands.
J Virol Methods. 1993 May;42(2-3):265-79. doi: 10.1016/0166-0934(93)90038-s.
Two sets of consensus PCR primers consisting of a common 3' primer CP-I and two 5'-primers, CP-IIG (primer set A) and CP-IIS (primer set B), in the E1 open reading frame of the human papillomavirus (HPV) genome are presented. These two primer sets enabled the detection of a 188 base pair (bp) fragment of HPV 1, 2, 3, 4, 5, 6b, 7, 8, 9, 10a, 11, 12, 14a, 16, 17, 18, 19, 20, 21, 22, 24, 25, 31, 33, 36, 37, 38, 39 and 46. HPV types 15, 23, 49 and 50 were poorly amplified and HPV type 41 was not amplified. The method is suitable for the detection of HPV DNA sequences in clinical samples of both cervical and cutaneous lesions.
本文介绍了两组用于人乳头瘤病毒（HPV）基因组E1开放阅读框的一致性聚合酶链式反应（PCR）引物，其中包括一个通用的3'引物CP-I以及两个5'引物CP-IIG（引物组A）和CP-IIS（引物组B）。这两组引物能够检测HPV 1、2、3、4、5、6b、7、8、9、10a、11、12、14a、16、17、18、19、20、21、22、24、25、31、33、36、37、38、39和46型的188个碱基对（bp）片段。HPV 15、23、49和50型扩增效果不佳，HPV 41型未扩增。该方法适用于检测宫颈和皮肤病变临床样本中的HPV DNA序列。
