利用聚合酶链反应和单个突变引物构建人乳头瘤病毒11型E5A蛋白的位点特异性突变体。
Construction of site-specific mutants of E5A protein of HPV-11 using the polymerase chain reaction and a single mutant primer.
作者信息
Chen S L, Han C P, Tsao Y P
机构信息
Department of Microbiology and Immunology, National Defense Medical Center, Taipei, Taiwan.
出版信息
J Virol Methods. 1993 Apr;42(1):89-94. doi: 10.1016/0166-0934(93)90179-u.
PMID:8391542
Abstract
The method of Perrin and Gilliland (1990) was modified to create site-specific mutants. The polymerase chain reaction and a single mutant primer are needed to carry out site-specific mutagenesis. Using this method, removal of the excess primers and nucleotides from the initial amplification is not necessary. This method provides a simpler way to generate site-specific mutants.
摘要
佩兰和吉利兰（1990年）的方法经过改良以创建位点特异性突变体。进行位点特异性诱变需要聚合酶链反应和一条单突变引物。使用这种方法，无需从初始扩增中去除多余的引物和核苷酸。该方法提供了一种更简单的生成位点特异性突变体的方式。
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