Arihara K, Cassens R G, Luchansky J B
Department of Food Microbiology and Toxicology, University of Wisconsin-Madison 53706.
Int J Food Microbiol. 1993 Jul;19(2):123-34. doi: 10.1016/0168-1605(93)90178-j.
Laboratory cultures and environmental isolates of bacteria were screened for antagonism towards Listeria monocytogenes using an agar spot test. Seven of the 163 strains that were tested, one Streptococcus bovis, one Enterococcus casseliflavus, two E. avium and three E. faecium, consistently displayed antilisterial activity. Cell-free, pH-neutralized supernatants prepared from the three E. faecium strains (JBL1061, JBL1083 and JBL1351) exhibited strong antilisterial activity against L. monocytogenes, and were subjected to more detailed analyses. The antagonistic factors produced by these three strains were sensitive to chloroform and several proteolytic enzymes, resistant to heat (121 degrees C, 20 min), and stable over a wide pH range (3.0-10.0). Moreover, they were listericidal without causing cell lysis. These data suggest that a bacteriocin(s) is involved in the inhibition of L. monocytogenes by E. faecium JBL1061, JBL1083 and JBL1351.
采用琼脂斑点试验，对细菌的实验室培养物和环境分离株进行了针对单核细胞增生李斯特菌的拮抗作用筛选。在测试的163株菌株中，有7株，即1株牛链球菌、1株格氏肠球菌、2株鸟肠球菌和3株粪肠球菌，始终表现出抗李斯特菌活性。从3株粪肠球菌菌株（JBL1061、JBL1083和JBL1351）制备的无细胞、pH值中和的上清液对单核细胞增生李斯特菌表现出强烈的抗李斯特菌活性，并对其进行了更详细的分析。这3株菌株产生的拮抗因子对氯仿和几种蛋白水解酶敏感，对热（121℃，20分钟）有抗性，并且在较宽的pH范围（3.0 - 10.0）内稳定。此外，它们具有杀李斯特菌作用但不引起细胞裂解。这些数据表明，粪肠球菌JBL1061、JBL1083和JBL1351对单核细胞增生李斯特菌的抑制作用涉及一种细菌素。
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