适用于体外成熟（IVM）和体外受精（IVF）来源的牛胚胎的灵敏性别鉴定分析方法。
Sensitive sex determination assay applicable to bovine embryos derived from IVM and IVF.
作者信息
Kirkpatrick B W, Monson R L
机构信息
Department of Meat and Animal Science, University of Wisconsin, Madison 53706.
出版信息
J Reprod Fertil. 1993 Jul;98(2):335-40. doi: 10.1530/jrf.0.0980335.
A rapid, sensitive method for sex determination which uses nested, allele-specific amplification of bovine zfx and zfy was developed. Accuracy of the sex determination assay was verified with lymphocyte-derived DNA from 24 cattle of known gender. Reliability and sensitivity of the assay in embryonic sex determination were examined by typing duplicate bovine embryo biopsies of 2-8 cells. Sex determination was possible in 38 of 40 biopsies, and agreement between biopsy assays occurred in 17 of 18 cases. Applicability of the sex determination assay was further verified by examining gender of 12 fetuses derived from sexed embryos. Embryonic and fetal gender determinations agreed in 11 of 12 cases with the twelfth yielding an ambiguous result from fetal ultrasound examination.
开发了一种快速、灵敏的性别鉴定方法，该方法利用牛zfx和zfy基因的巢式、等位基因特异性扩增。用来自24头已知性别的牛的淋巴细胞衍生DNA验证了性别鉴定试验的准确性。通过对2-8个细胞的重复牛胚胎活检进行分型，检测了该试验在胚胎性别鉴定中的可靠性和敏感性。40次活检中有38次能够进行性别鉴定，18例中有17例活检试验结果一致。通过检查12个来自经性别鉴定胚胎的胎儿的性别，进一步验证了性别鉴定试验的适用性。12例中有11例胚胎和胎儿性别鉴定结果一致，第12例胎儿超声检查结果不明确。
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