Wood R, Dong H, Katzenstein D A, Merigan T C
Division of Infectious Diseases, Stanford University Medical Center, CA 94305.
J Acquir Immune Defic Syndr (1988). 1993 Mar;6(3):237-40.
HIV proviral load was determined by quantitative DNA polymerase chain reaction (PCR) in peripheral blood mononuclear cells (PBMC) and lymphocyte subsets isolated by cell sorter. Provirus measured in PBMC, when expressed as HIV copy number per million CD4+ cells, resulted in values which approximated those obtained from sorted CD4+ T lymphocytes. A cross sectional analysis of HIV proviral load in CD4+ T cells from 25 previously untreated and 30 zidovudine-treated seropositive patients with CD4+ T-cell counts between 25 and 802/mm3 demonstrated HIV copy numbers ranging from 1 copy per 10,000 cells in early disease to 1 copy per 10 cells in advanced disease. HIV proviral load can be rapidly assayed by PCR to give a reproducible value which varies over a 1,000-fold range and is positively correlated with cell infectivity as measured by a quantitative micrococulture assay. A less technically demanding assay using PBMC as substrate can give similar results to those obtained with sorted CD4+ T cells.
通过定量DNA聚合酶链反应（PCR）测定外周血单核细胞（PBMC）以及通过细胞分选仪分离出的淋巴细胞亚群中的HIV前病毒载量。在PBMC中测得的前病毒，以每百万CD4+细胞中的HIV拷贝数表示时，其结果与从分选的CD4+ T淋巴细胞中获得的结果相近。对25例未经治疗和30例接受齐多夫定治疗的血清阳性患者的CD4+ T细胞中的HIV前病毒载量进行横断面分析，这些患者的CD4+ T细胞计数在25至802/mm3之间，结果显示HIV拷贝数从疾病早期的每10,000个细胞1拷贝到疾病晚期的每10个细胞1拷贝不等。可通过PCR快速检测HIV前病毒载量，以获得一个可重复的值，该值在1000倍的范围内变化，并且与通过定量微培养测定法测得的细胞感染性呈正相关。使用PBMC作为底物的技术要求较低的检测方法可得到与分选的CD4+ T细胞检测结果相似的结果。
