体内紫外线诱导的DNA-蛋白质交联显示，两种非组蛋白与活性hsp-70基因的启动子区域相关联，组蛋白H1与转录区域相关联。

Belikov S V, Belgovsky A I, Preobrazhenskaya O V, Karpov V L, Mirzabekov A D

W.A. Engelhardt Institute of Molecular Biology, Russian Academy of Sciences, Moscow.

Nucleic Acids Res. 1993 Feb 25;21(4):1031-4. doi: 10.1093/nar/21.4.1031.

We described here an approach for mapping proteins on any sequence of genomic DNA. UV-induced DNA-protein crosslinking within whole cells and the 'protein image' hybridization technique (1) were applied to test the proteins bound to different regions of the D. melanogaster hsp-70 gene. The histone H1-DNA association with the coding region is shown to be maintained, even during very intensive transcription, but is absent in the promoter. Two non-histone proteins with apparent molecular masses of 50 kD (p50) and 100 kD (p100) are crosslinked only to the active hsp-70 gene regulatory region and preferentially bind to its complementary and coding DNA strands, respectively.

我们在此描述了一种在任何基因组DNA序列上绘制蛋白质图谱的方法。将全细胞内紫外线诱导的DNA-蛋白质交联和“蛋白质图像”杂交技术（1）应用于测试与黑腹果蝇hsp-70基因不同区域结合的蛋白质。结果表明，即使在非常活跃的转录过程中，组蛋白H1与编码区的DNA结合仍得以维持，但在启动子区域则不存在这种结合。两种表观分子量分别为50 kD（p50）和100 kD（p100）的非组蛋白仅与活跃的hsp-70基因调控区域发生交联，且分别优先结合其互补DNA链和编码DNA链。