Biosynthesis of 12(S)-hydroxyeicosatetraenoic acid by bovine corneal epithelium.
作者信息
Oliw E H
机构信息
Department of Pharmaceutical Pharmacology, Uppsala University Biomedical Centre, Sweden.
出版信息
Acta Physiol Scand. 1993 Jan;147(1):117-21. doi: 10.1111/j.1748-1716.1993.tb09479.x.
Abstract
Microsomes of bovine corneal epithelium metabolized [14C]arachidonic acid extensively to [14C]12-hydroxyeicosatetraenoic acid. The biosynthesis was not stimulated by exogenous NADPH and it could be inhibited by over 90% with two lipoxygenase inhibitors, esculetin and eicosatetraynoic acid. Chiral phase high performance liquid chromatography showed that 12-HETE consisted of the 12S enantiomer. The results indicate biosynthesis by 12S-lipoxygenase of bovine corneal epithelium rather than by cytochrome P450.
摘要
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