马肝醇脱氢酶催化醛的氧化反应。

The oxidation of aldehydes by horse liver alcohol dehydrogenase.

作者信息

Henehan G T, Kenyon G L, Oppenheimer N J

机构信息

Department of Pharmaceutical Chemistry, University of California, San Francisco 94143-0446.

出版信息

Adv Exp Med Biol. 1993;328:481-91. doi: 10.1007/978-1-4615-2904-0_51.

DOI:10.1007/978-1-4615-2904-0_51

PMID:8493927

Abstract

A lag phase in the spectrophotometric assay progress curve of aldehyde oxidation by HL-ADH was observed and characterised. The aldehyde oxidation and aldehyde dismutation reactions were shown to be related, and a mechanism to explain net aldehyde oxidation was proposed. The spectrophotometric assay was shown to be unsuitable for measurement of kinetic parameters for aldehyde oxidation by HL-ADH, and kinetic constants previously determined were shown to be in error. Existing data on the aldehyde dismutation reaction are insufficient to discount a role for HL-ADH in aldehyde transformation in vivo.

摘要

观察并表征了HL-ADH催化醛氧化的分光光度法测定过程曲线中的延迟期。醛氧化和醛歧化反应被证明是相关的，并提出了一种解释醛净氧化的机制。分光光度法测定被证明不适用于测量HL-ADH催化醛氧化的动力学参数，先前测定的动力学常数被证明是错误的。关于醛歧化反应的现有数据不足以排除HL-ADH在体内醛转化中的作用。