The synthesis of polyadenylated RNA and other RNA species in synchronised CHK cells.

Chinese hamster kidney cells have been synchronised by the double thymidine block technique. The rate of incorporation of [5-3H]uridine into the polyadenylated RNA fraction has been analysed throughout two complete cell cycles and compared with the rate of incorporation into other RNA species and the UTP pool. In contrast with other reports, we have found that synthesis of 18 S rRNA continues steadily throughout the cell cycle, as does synthesis of 5 S RNA and tRNA. In the case of the polyadenylated RNA, however, our data indicate a major peak of synthesis during S phase and a smaller peak coinciding with G1.