通过拓扑异构酶II介导的DNA环切除方案对果蝇X染色体500千碱基区域的基因组DNA环组织进行图谱绘制。
Mapping of genomic DNA loop organization in a 500-kilobase region of the Drosophila X chromosome by the topoisomerase II-mediated DNA loop excision protocol.
作者信息
Iarovaia O, Hancock R, Lagarkova M, Miassod R, Razin S V
机构信息
International Centre for Genetic Engineering and Biotechnology, Trieste, Italy.
出版信息
Mol Cell Biol. 1996 Jan;16(1):302-8. doi: 10.1128/MCB.16.1.302.
Abstract
The recently developed procedure of chromosomal DNA loop excision by topoisomerase II-mediated DNA cleavage at matrix attachment sites (S. V. Razin, R. Hancock, O. Iarovaia, O. Westergaard, I. Gromova, and G. P. Georgiev, Cold Spring Harbor Symp. Quant. Biol. 58:25-35, 1993; I. I. Gromova, B. Thompsen, and S. V. Razin, Proc. Natl. Acad. Sci. USA 92:102-106, 1995) has been employed for mapping the DNA loop anchorage sites in a 500-kb region of the Drosophila melanogaster X chromosome. Eleven anchorage sites delimiting 10 DNA loops ranging in size from 20 to 90 kb were found within this region. Ten of these 11 anchorage sites colocalize with previously mapped scaffold attachment regions. However, a number of other scaffold attachment regions are found to be located in loop DNA.
摘要
最近开发的一种程序，即通过拓扑异构酶II介导的在基质附着位点处的DNA切割来切除染色体DNA环（S. V. 拉津、R. 汉考克、O. 亚罗瓦亚、O. 韦斯特加德、I. 格罗莫娃和G. P. 乔治耶夫，《冷泉港定量生物学研讨会论文集》58:25 - 35，1993；I. I. 格罗莫娃、B. 汤普森和S. V. 拉津，《美国国家科学院院刊》92:102 - 106，1995），已被用于绘制黑腹果蝇X染色体500 kb区域内的DNA环锚定位点。在该区域内发现了11个锚定位点，界定了10个大小从20到90 kb不等的DNA环。这11个锚定位点中的10个与先前绘制的支架附着区域共定位。然而，发现许多其他支架附着区域位于环DNA中。
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