电喷雾质谱法证明天然猪胃蛋白酶A中存在两种主要变体。
Electrospray mass spectrometric evidence for the occurrence of two major variants in native pig pepsin A.
作者信息
Green B N, Jones A T, Roberts N B
机构信息
VG Organio, Altrincham, Cheshire, U.K.
出版信息
Biochem J. 1996 Jan 1;313 ( Pt 1)(Pt 1):241-4. doi: 10.1042/bj3130241.
Native pig pepsin was analysed by negative ion electrospray mass spectrometry in order to rationalize anomalies between the published sequences. Outstanding variations in otherwise identical sequences indicate that amino acid residue 242 is either Asp or Tyr, and in some determinations an additional Ile is inserted at position 230. Mass spectrometric evidence is consistent with the presence, in the native enzyme, of two variants in comparable abundance, with either Asp or Tyr at residue 242. There is no evidence for the additional Ile at position 230.
为了合理解释已发表序列之间的异常情况，采用负离子电喷雾质谱法对天然猪胃蛋白酶进行了分析。在其他方面相同的序列中存在显著差异，表明氨基酸残基242要么是天冬氨酸（Asp）要么是酪氨酸（Tyr），并且在某些测定中，在位置230处额外插入了一个异亮氨酸（Ile）。质谱证据与天然酶中存在两种丰度相当的变体一致，残基242处为Asp或Tyr。没有证据表明在位置230处存在额外的Ile。
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