丙型肝炎病毒的宿主依赖性变异：系统发育分析

Host dependent variation of hepatitis C virus: phylogenetic analyses.

作者信息

Maekawa S, Enomoto N, Kurosaki M, Marumo F, Sato C

机构信息

Second Department of Internal Medicine, Tokyo Medical and Dental University, Japan.

出版信息

Arch Virol. 1995;140(12):2123-33. doi: 10.1007/BF01323235.

DOI:10.1007/BF01323235

PMID:8572936

Abstract

Hepatitis C virus quasispecies in six patients from three families were separated by single strand conformation polymorphism analysis and by determination of nucleotide sequences of envelope regions containing the E1 gene segment and hypervariable region-1 of each quasispecies. Four of the six patients had multiple quasispecies. Phylogenetic analyses indicated that all quasispecies from one individual were highly homologous to each other. The homology was higher in the E1 gene segment than in hypervariable region-1. Furthermore, all quasispecies found in members of one family (husband and wife) were also highly homologous, suggesting direct intrafamilial transmission. The direction of hepatitis C virus variation in hypervariable region-1, however, seems to differ depending on the host in intrafamilial transmission.

摘要

通过单链构象多态性分析以及对包含每个准种E1基因片段和高变区-1的包膜区域进行核苷酸序列测定，分离出了来自三个家庭的六名患者体内的丙型肝炎病毒准种。六名患者中有四名具有多个准种。系统发育分析表明，来自同一个体的所有准种彼此高度同源。E1基因片段中的同源性高于高变区-1中的同源性。此外，在一个家庭（丈夫和妻子）成员中发现的所有准种也高度同源，提示存在家庭内直接传播。然而，在家庭内传播中，高变区-1中丙型肝炎病毒的变异方向似乎因宿主不同而有所差异。