MHC I类B等位基因单肽复合物的产生与结晶
Production and crystallization of MHC class I B allele single peptide complexes.
作者信息
Reid S W, Smith K J, Jakobsen B K, O'Callaghan C A, Reyburn H, Harlos K, Stuart D I, McMichael A J, Bell J I, Jones E Y
机构信息
Laboratory of Molecular Biophysics, Oxford, UK.
出版信息
FEBS Lett. 1996 Mar 25;383(1-2):119-23. doi: 10.1016/0014-5793(96)00226-8.
Major histocompatibility complex class I B alleles, HLA B8, B53 and B3501 have been cloned, expressed, refolded and crystallized in specific complexes with a number of different 8-mer and 9-mer peptides. For some of these crystallization was initiated by cross-seeding between different B allele complexes. All crystallize in the space group P212121, with similar unit cell dimensions of approximately 52 A X 81 A X 112 A, contain one complex per asymmetric unit and diffract to approximately 2.0 A resolution.
主要组织相容性复合体I类B等位基因，即HLA B8、B53和B3501，已被克隆、表达、重折叠并与多种不同的8聚体和9聚体肽形成特定复合物后进行结晶。对于其中一些，结晶是通过不同B等位基因复合物之间的交叉接种启动的。所有这些都在空间群P212121中结晶，具有相似的晶胞尺寸，约为52 Å×81 Å×112 Å，每个不对称单元包含一个复合物，衍射分辨率约为2.0 Å。
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