编码小鼠ATBF1转录因子的cDNA的克隆

Cloning of the cDNA encoding the mouse ATBF1 transcription factor.

作者信息

Ido A, Miura Y, Watanabe M, Sakai M, Inoue Y, Miki T, Hashimoto T, Morinaga T, Nishi S, Tamaoki T

机构信息

Department of Medical Biochemistry, University of Calgary, Alberta, Canada.

出版信息

Gene. 1996 Feb 12;168(2):227-31. doi: 10.1016/0378-1119(95)00740-7.

DOI:10.1016/0378-1119(95)00740-7

PMID:8654949

Abstract

We have isolated a mouse ATBF1 cDNA which is 12-kb long and capable of encoding a 406-kDa protein containing four homeodomains and 23 zinc-finger motifs. Mouse ATBF1 is 94% homologous to the human ATBF1-A transcription factor. Northern blot and RNase protection analysis showed that levels of ATBF1 transcripts were low in adult mouse tissues, but high in developing brain, consistent with a role for ATBF1 in neuronal differentiation.

摘要

我们分离出了一个小鼠ATBF1 cDNA，其长度为12 kb，能够编码一种406 kDa的蛋白质，该蛋白质含有四个同源结构域和23个锌指基序。小鼠ATBF1与人类ATBF1-A转录因子的同源性为94%。Northern印迹和核糖核酸酶保护分析表明，ATBF1转录本在成年小鼠组织中的水平较低，但在发育中的大脑中水平较高，这与ATBF1在神经元分化中的作用一致。