小鼠垂体腺苷酸环化酶激活多肽受体的cDNA克隆
cDNA cloning of a mouse pituitary adenylate cyclase-activating polypeptide receptor.
作者信息
Hashimoto H, Yamamoto K, Hagigara N, Ogawa N, Nishino A, Aino H, Nogi H, Imanishi K, Matsuda T, Baba A
机构信息
Department of Pharmacology, Faculty of Pharmaceutical Sciences, Osaka University, Japan.
出版信息
Biochim Biophys Acta. 1996 Jun 11;1281(2):129-33. doi: 10.1016/0005-2736(96)00056-9.
A cDNA clone for mouse pituitary adenylate cyclase-activating polypeptide (PACAP) receptor (PACAP-R) was obtained from the brain using reverse transcription-polymerase chain reaction (RT-PCR). The recombinant PACAP receptor expressed in COS cells bound PACAP with about 1000-times higher affinity than vasoactive intestinal polypeptide (VIP), and PACAP stimulated adenylate cyclase through the cloned PACAP receptor. The mouse PACAP receptor consists of 496 amino acids, contains seven transmembrane segments and has 98.4%, 93.0%, and 92.5% identity with the rat, bovine, and human PACAP-R, respectively.
利用逆转录-聚合酶链反应（RT-PCR）从鼠脑获得了小鼠垂体腺苷酸环化酶激活多肽（PACAP）受体（PACAP-R）的cDNA克隆。在COS细胞中表达的重组PACAP受体与PACAP结合的亲和力比血管活性肠肽（VIP）高约1000倍，并且PACAP通过克隆的PACAP受体刺激腺苷酸环化酶。小鼠PACAP受体由496个氨基酸组成，含有7个跨膜区段，与大鼠、牛和人PACAP-R的同源性分别为98.4%、93.0%和92.5%。
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