Wang H, Li K Y, Wu G C, Cao X D
State Laboratory of Medical Neurobiology, Shanghai Medical University, People's Republic of China.
Acupunct Electrother Res. 1995 Aug-Dec;20(3-4):163-72. doi: 10.3727/036012995816357087.
The present study was to trace the neuronal pathway in the spinal cord and brainstem following noxious stimulation and acupuncture plus noxious stimulation by using immunohistochemical technique to detect the expression of c-fos like protein. The results showed that in both the noxious stimulation group and acupuncture of Zusanli(St.36) and Lanwei(Extra 33) points plus noxious stimulation group a lot of Fos-like immunoreactive (FLI) cell nuclei appeared in the following structures: dorsal horn of lumbar and sacral spinal cord (Laminae I, II, V and X), n. reticularis lateralis, n. reticularis paragigantocellularis lateralis, area A1, n. tractus solitarii, n. raphe magnus, locus coeruleus, n. raphe dorsalis and the periaqueductal grey. In the control group no obvious c-fos expression was shown in the structures mentioned above. It was also shown that in the lumbar and sacral spinal cord the number of FLI nuclei in the acupuncture plus noxious stimulation group was more than that in the noxious stimulation group, but in locus coeruleus and the ventral part of the periaqueductal grey the number of FLI nuclei in the acupuncture plus noxious stimulation group was less than that of the noxious stimulation group. The results imply that acupuncture and noxious stimulation may activate the intrinsic pain modulating system through the spinal dorsal horn, then suppress the c-fos expression induced by noxious stimulation in the locus coeruleus and ventral part of the periaqueductal grey.
本研究旨在通过免疫组织化学技术检测c-fos样蛋白的表达，追踪有害刺激以及针刺加有害刺激后脊髓和脑干中的神经通路。结果显示，在有害刺激组以及针刺足三里（St.36）和阑尾（Extra 33）穴加有害刺激组中，在以下结构中出现了许多Fos样免疫反应性（FLI）细胞核：腰骶脊髓背角（I、II、V和X层）、外侧网状核、外侧旁巨细胞网状核、A1区、孤束核、中缝大核、蓝斑核、中缝背核和导水管周围灰质。在对照组中，上述结构未显示明显的c-fos表达。还显示，在腰骶脊髓中，针刺加有害刺激组的FLI细胞核数量多于有害刺激组，但在蓝斑核和导水管周围灰质腹侧部分，针刺加有害刺激组的FLI细胞核数量少于有害刺激组。结果表明，针刺和有害刺激可能通过脊髓背角激活内源性疼痛调制系统，进而抑制有害刺激在蓝斑核和导水管周围灰质腹侧部分诱导的c-fos表达。
