吗啡对体外培养的小鼠腹腔巨噬细胞白细胞介素-1及肿瘤坏死因子α生成的影响。
Effect of morphine on interleukin-1 and tumor necrosis factor alpha production from mouse peritoneal macrophages in vitro.
作者信息
Bian T H, Wang X F, Li X Y
机构信息
Shanghai Institute of Materia Medica, Chinese Academy of Sciences, China.
出版信息
Zhongguo Yao Li Xue Bao. 1995 Sep;16(5):449-51.
AIM
To study the influence of morphine on lymphokine production from mouse peritoneal macrophages (PM phi).
METHODS
The productions of interleukin-1 (IL-1) and tumor necrosis factor (TNF-alpha) from PM phi under various concentrations of morphine with or without naloxone were measured by testing thymocyte proliferation and L929 cell lysis in vitro.
RESULTS
Both morphine (1 x 10(-8) - 1 x 10(-3) mol L-1) and naloxone (50 mumol L-1) inhibited IL-1 production from mouse PM phi primed by sodium thioglycolate. Naloxone did not block the inhibitory effect of morphine. TNF-alpha production from PM phi was inhibited by only high concentration (1 mmol L-1) of morphine which was not blocked by naloxone.
CONCLUSION
Morphine had a direct inhibitory effect on PM phi, which was not mediated by opioid receptor of PM phi.
目的
研究吗啡对小鼠腹腔巨噬细胞（PM phi）产生淋巴因子的影响。
方法
通过体外检测胸腺细胞增殖和L929细胞裂解，测定在不同浓度吗啡存在或不存在纳洛酮的情况下PM phi产生白细胞介素-1（IL-1）和肿瘤坏死因子（TNF-α）的情况。
结果
吗啡（1×10⁻⁸ - 1×10⁻³ mol L⁻¹）和纳洛酮（50 μmol L⁻¹）均抑制经巯基乙酸钠致敏的小鼠PM phi产生IL-1。纳洛酮不能阻断吗啡的抑制作用。仅高浓度（1 mmol L⁻¹）的吗啡抑制PM phi产生TNF-α，且该作用不被纳洛酮阻断。
结论
吗啡对PM phi有直接抑制作用，该作用不是由PM phi的阿片受体介导的。
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