环磷酸腺苷、钙和蛋白激酶C介导的途径对支持细胞中c-fos信使核糖核酸表达的调控

Regulation of c-fos mRNA expression in Sertoli cells by cyclic AMP, calcium, and protein kinase C mediated pathways.

作者信息

Jia M C, Ravindranath N, Papadopoulos V, Dym M

机构信息

Department of Cell Biology, Georgetown University Medical Center, Washington, DC 20007, USA.

出版信息

Mol Cell Biochem. 1996 Mar 9;156(1):43-9. doi: 10.1007/BF00239318.

DOI:10.1007/BF00239318

PMID:8709975

Abstract

The role of second messenger pathways, cyclic AMP, calcium, and protein kinase C (PKC) in the transcriptional regulation of c-fos protooncogene expression in rat Sertoli cells was investigated. c-fos expression was monitored by Northern blot analysis. Although the action of FSH on Sertoli cells is considered to be mediated by cAMP, dibutyryl cAMP (dbcAMP), a potent membrane permeable analog of cAMP, induced much less c-fos mRNA expression than FSH ( < 50%) suggesting that additional cAMP-independent mechanisms may mediate the effect of FSH on c-fos. Specific intracellular inhibitors of PKC decreased c-fos induction in response to FSH by more than 50%. Ionomycin, which increases intracellular free calcium concentration, induced c-fos expression significantly. These data demonstrate that Sertoli cell c-fos mRNA expression is under multifactorial regulation by cAMP, calcium, and PKC.

摘要

研究了第二信使途径、环磷酸腺苷（cAMP）、钙和蛋白激酶C（PKC）在大鼠支持细胞中c-fos原癌基因表达转录调控中的作用。通过Northern印迹分析监测c-fos表达。尽管促卵泡激素（FSH）对支持细胞的作用被认为是由cAMP介导的，但二丁酰环磷腺苷（dbcAMP），一种有效的可透过细胞膜的cAMP类似物，诱导的c-fos mRNA表达比FSH少得多（<50%），这表明可能存在其他不依赖cAMP的机制介导FSH对c-fos的作用。PKC的特异性细胞内抑制剂使FSH诱导的c-fos减少超过50%。离子霉素可增加细胞内游离钙浓度，能显著诱导c-fos表达。这些数据表明，支持细胞c-fos mRNA表达受cAMP、钙和PKC的多因素调控。