埃及空肠弯曲菌和/或结肠弯曲菌分离株鞭毛蛋白基因的限制性片段长度多态性
Restriction fragment length polymorphism of flagellin genes of Campylobacter jejuni and/or C. coli isolates from Egypt.
作者信息
Mohran Z S, Guerry P, Lior H, Murphy J R, el-Gendy A M, Mikhail M M, Oyofo B A
机构信息
U.S. Naval Medical Research Unit Number 3, Cairo, Egypt.
出版信息
J Clin Microbiol. 1996 May;34(5):1216-9. doi: 10.1128/jcm.34.5.1216-1219.1996.
The conservation of flagellin genes from thermophilic Campylobacter spp. strains isolated in Egypt was evaluated by a restriction fragment length polymorphism (RFLP) assay. The flaA and flaB genes were amplified from 59 independent clinical isolates and digested with EcoRI and PstI, and the resulting patterns were compared with each other and with previously described RFLP groups. The results indicate that the isolates fell into 14 groups for flaA and 11 groups for flaB, 9 of which have been described, and that considerable genetic variability exists among isolates belonging to the same LIO serogroup. In most cases, the flaB gene displayed the same RFLP pattern as that of the flaA gene of the same strain, although some variability was observed. The data suggest that more variability of flagellin genes exists within the LIO serogroups common to Campylobacter field isolates from Egypt than has previously been reported for North American isolates.
通过限制性片段长度多态性（RFLP）分析评估了从埃及分离的嗜热弯曲杆菌菌株中鞭毛蛋白基因的保守性。从59个独立的临床分离株中扩增出flaA和flaB基因，并用EcoRI和PstI进行消化，将所得图谱相互比较，并与先前描述的RFLP组进行比较。结果表明，分离株的flaA基因分为14组，flaB基因分为11组，其中9组已被描述，并且属于同一LIO血清群的分离株之间存在相当大的遗传变异性。在大多数情况下，flaB基因显示出与同一菌株的flaA基因相同的RFLP图谱，尽管观察到了一些变异性。数据表明，与之前报道的北美分离株相比，埃及弯曲杆菌野外分离株常见的LIO血清群中鞭毛蛋白基因存在更多变异性。
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