Lowe B S, Jeffa N K, New L, Pedersen C, Engbaek K, Marsh K
Kenya Medical Research Institute, CRC Kilifi Unit, Kenya.
Trans R Soc Trop Med Hyg. 1996 Jan-Feb;90(1):34-6. doi: 10.1016/s0035-9203(96)90470-8.
Traditional Giemsa-stained thick blood films were compared with 2 fluorescence microscopy techniques, acridine orange (AO) staining of thin blood films and the quantitative buffy coat (QBC) method, for the microscopical diagnosis of malaria. Of 200 samples examined, 141 were positive by Giemsa staining, 146 by AO and 137 by QBC. Overall sensitivities for the 2 fluorescence techniques compared to Giemsa staining were good: AO 97.9% and QBC 93.6%. However, with parasitaemias < 100/microL the QBC sensitivity fell to 41.7% whereas that of AO was 83.3%. Both AO and QBC were unable to differentiate accurately between individual malaria species. We conclude that the QBC technique alone cannot replace Giemsa-stained thick blood films for most purposes in an African setting. However, apart from species differentiation, the AO method is an appropriate technique for the laboratory diagnosis of malaria in developing countries.
将传统吉姆萨染色厚血膜与两种荧光显微镜技术（薄血膜吖啶橙染色法和定量血沉棕黄层法）进行比较，用于疟疾的显微镜诊断。在检测的200份样本中，吉姆萨染色法检测出141份阳性，吖啶橙染色法检测出146份阳性，定量血沉棕黄层法检测出137份阳性。与吉姆萨染色法相比，两种荧光技术的总体敏感性良好：吖啶橙染色法为97.9%，定量血沉棕黄层法为93.6%。然而，当疟原虫血症<100/μL时，定量血沉棕黄层法的敏感性降至41.7%，而吖啶橙染色法为83.3%。吖啶橙染色法和定量血沉棕黄层法均无法准确区分各个疟原虫种类。我们得出结论，在非洲环境下，仅定量血沉棕黄层法在大多数情况下不能替代吉姆萨染色厚血膜。然而，除了疟原虫种类区分外，吖啶橙染色法是发展中国家疟疾实验室诊断的一种合适技术。
