利用印度新德里急性腹泻患儿的样本对轮状病毒进行G型和P型分类。
Classification of rotavirus into G and P types with specimens from children with acute diarrhea in New Delhi, India.
作者信息
Husain M, Seth P, Dar L, Broor S
机构信息
Department of Microbiology, All India Institute of Medical Sciences, New Delhi, India.
出版信息
J Clin Microbiol. 1996 Jun;34(6):1592-4. doi: 10.1128/jcm.34.6.1592-1594.1996.
Abstract
Sixty rotavirus-positive stool specimens from children with diarrhea were classified into G and P genotypes. G typing was done by PCR and then by hybridization with G type-specific (G1 to G4) oligonucleotide probes, whereas nested PCR was performed for P typing. Thirty-nine samples could be classified into both G and P types, of which P8G1 and P4G2 (33% each) genotypes were predominant. The P6 genotype was detected in four children with diarrhea.
摘要
从腹泻儿童中采集的60份轮状病毒阳性粪便样本被分为G型和P型基因型。G型分型通过聚合酶链反应（PCR）进行，然后与G型特异性（G1至G4）寡核苷酸探针杂交，而P型分型则采用巢式PCR。39个样本可同时分为G型和P型，其中P8G1和P4G2基因型（各占33%）占主导地位。在4名腹泻儿童中检测到了P6基因型。
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