亚硒酸钠和硒代蛋氨酸对砷诱导的人外周血淋巴细胞遗传毒性的保护作用
[Protective effects of sodium selenite and selenomethionine on genotoxicity to human peripheral lymphocytes induced by arsenic].
作者信息
Hu G, Liu X, Liu J
机构信息
Cancer Institute, Chinese Academy of Medical Sciences, Peking Union Medical College, Beijing.
出版信息
Zhonghua Yu Fang Yi Xue Za Zhi. 1996 Jan;30(1):26-9.
Inhibition bioassay of sister chromatid exchanges (SCE) and DNA synthesis was carried out to study the mechanism that selenium, as a chemical agent for intervention, could antagonize the effects of arsenic on body damage and lower incidence of tumor. Results demonstrated that SCE frequency in peripheral lymphocytes induced by As2O3 could be significantly reduced by preincubation with 5 x 10(-7), 1 x 10(-6) mol/L Na2SeO3 and co-exposure of 1 x 10(-6) mol/L selenomethionine. Inhibition of DNA synthesis could be antagonized by preincubation in with 5 x 10(-6) mol/L Na2SeO3 and co-exposure of 1 x 10(-6), 5 x 10(-6) mol/L selenomethionine. But, the mechanism that protective effects of selenium on the damage to cell genetic material induced by arsenic should be studied further.
进行了姐妹染色单体交换（SCE）抑制生物测定和DNA合成实验，以研究作为干预化学剂的硒拮抗砷对机体损害及降低肿瘤发生率的作用机制。结果表明，用5×10⁻⁷、1×10⁻⁶mol/L亚硒酸钠预孵育以及与1×10⁻⁶mol/L硒代蛋氨酸共同暴露，可显著降低As₂O₃诱导的外周血淋巴细胞SCE频率。用5×10⁻⁶mol/L亚硒酸钠预孵育以及与1×10⁻⁶、5×10⁻⁶mol/L硒代蛋氨酸共同暴露，可拮抗DNA合成的抑制。但是，硒对砷诱导的细胞遗传物质损伤的保护作用机制尚需进一步研究。
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