针对新型隐球菌血清型D特异性抗原8的凝集性单克隆抗体的产生。
Production of agglutinating monoclonal antibody against antigen 8 specific for Cryptococcus neoformans serotype D.
作者信息
Ikeda R, Nishimura S, Nishikawa A, Shinoda T
机构信息
Department of Microbiology, Meiji College of Pharmacy, Tokyo, Japan.
出版信息
Clin Diagn Lab Immunol. 1996 Jan;3(1):89-92. doi: 10.1128/cdli.3.1.89-92.1996.
A hybridoma (clone CRND-8) that produced agglutinating monoclonal antibody (MAb) against Cryptococcus neoformans serotype D was established by using a soluble capsular polysaccharide-keyhole limpet hemocyanin conjugate for immunization. The isotype was immunoglobulin M(kappa). Specificity was determined by cell slide agglutination and enzyme-linked immunosorbent assay (ELISA). In both tests, the MAb reacted to serotypes D and A-D but not to serotypes A, B, and C. Furthermore, the specificity of the MAb determined by ELISA was the same as that of polyclonal antibody factor serum (PAb factor) 8, which showed high-level reactivity with serotypes D and A-D. These results supported the deduced specificity of the PAb-based antigenic factor 8. A total of 15 isolates of serotypes D and A-D but no serotype A isolates reacted with the MAb in cell slide agglutination tests. CRND-8 MAb can be used in place of PAb factor 8 for serotyping C. neoformans isolates and for the analysis of the antigen 8 epitope.
通过使用可溶性荚膜多糖-钥孔戚血蓝蛋白偶联物进行免疫，建立了一株产生抗新型隐球菌D血清型凝集单克隆抗体（MAb）的杂交瘤（克隆CRND-8）。其同种型为免疫球蛋白M（κ）。通过细胞玻片凝集试验和酶联免疫吸附测定（ELISA）确定特异性。在这两种试验中，该MAb与D血清型以及A-D血清型反应，但不与A、B和C血清型反应。此外，通过ELISA确定的MAb特异性与多克隆抗体因子血清（PAb因子）8相同，后者与D血清型以及A-D血清型表现出高水平反应性。这些结果支持了基于PAb的抗原因子8的推导特异性。在细胞玻片凝集试验中，共有15株D血清型和A-D血清型分离株与该MAb反应，但没有A血清型分离株反应。CRND-8 MAb可用于替代PAb因子8对新型隐球菌分离株进行血清分型以及分析抗原8表位。
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