介电常数和离子强度对DNA沉淀的影响。
Dielectric constant and ionic strength effects on DNA precipitation.
作者信息
Flock S, Labarbe R, Houssier C
机构信息
Laboratoire de Chimie Macromoléculaire et Chimie Physique, Université de Liège, Belgium.
出版信息
Biophys J. 1996 Mar;70(3):1456-65. doi: 10.1016/S0006-3495(96)79705-9.
Abstract
We have investigated the effect of different zwitterionic compounds on DNA precipitation induced by spermine4+. Glycine, beta-alanine, 4-aminobutyric acid, and 6-aminocaproic acid have shown an increasing capacity to attenuate DNA precipitation. This protection effect has been correlated with the dielectric constant increase of their corresponding solutions. Calculations based on these experimental data and counter-ion condensation theory have confirmed the importance of this parameter for DNA-ion interactions and precipitation mechanisms. We have also observed a resolubilization of DNA in the presence of 6-aminocaproic acid at high spermine4+ concentration and in the presence of glycine at high spermidine3+ concentration. This could be explained by an increase of screening effect with polyamine concentration.
摘要
我们研究了不同两性离子化合物对精胺4+诱导的DNA沉淀的影响。甘氨酸、β-丙氨酸、4-氨基丁酸和6-氨基己酸表现出越来越强的减弱DNA沉淀的能力。这种保护作用与它们相应溶液的介电常数增加有关。基于这些实验数据和抗衡离子凝聚理论的计算证实了该参数对于DNA-离子相互作用和沉淀机制的重要性。我们还观察到，在高浓度精胺4+存在下以及在高浓度亚精胺3+存在下，6-氨基己酸存在时DNA会重新溶解，甘氨酸存在时DNA也会重新溶解。这可以用多胺浓度增加导致的屏蔽效应增强来解释。
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