在兽医寄生虫学中使用分子工具进行诊断。

Using molecular tools for diagnosis in veterinary parasitology.

作者信息

Comes A M, Humbert J F, Cabaret J, Elard L

机构信息

Centre de recherches de Tours, Inra, Nouzilly, France.

出版信息

Vet Res. 1996;27(4-5):333-42.

PMID:8822605

Abstract

The development of molecular biology has made available tools that identify parasites which are important in veterinary parasitology. PCR (polymerase chain reaction) is the most frequently used tool. Target sequences are ribosomal DNA and RNA as well as fragments derived from RAPD (random amplified polymorphic DNA). The most studied protozoan genera have been: Eimeria, Babesia, Theileria, Trypanosoma and Cryptosporidium. Trichinella species and ruminant Trichostrongylid parasites have been the most investigated helminths. The use of molecular tools for identification in veterinary parasitology, however, remains a research technique rather than one that is currently used in the field for diagnostic purposes.

摘要

分子生物学的发展提供了可用于鉴定在兽医寄生虫学中重要寄生虫的工具。聚合酶链反应（PCR）是最常用的工具。靶序列是核糖体DNA和RNA以及源自随机扩增多态性DNA（RAPD）的片段。研究最多的原生动物属有：艾美耳属、巴贝斯属、泰勒虫属、锥虫属和隐孢子虫属。旋毛虫属物种和反刍动物毛圆线虫寄生虫是研究最多的蠕虫。然而，在兽医寄生虫学中使用分子工具进行鉴定仍然是一种研究技术，而非目前在该领域用于诊断目的的技术。