孤啡肽/痛敏肽免疫反应性神经纤维与大鼠脊髓中含内源性阿片类物质的神经纤维平行。

Orphanin FQ/nociceptin-immunoreactive nerve fibers parallel those containing endogenous opioids in rat spinal cord.

作者信息

Riedl M, Shuster S, Vulchanova L, Wang J, Loh H H, Elde R

机构信息

Department of Cell Biology and Neuroanatomy, University of Minnesota, Minneapolis 55455, USA.

出版信息

Neuroreport. 1996 May 31;7(8):1369-72. doi: 10.1097/00001756-199605310-00007.

DOI:10.1097/00001756-199605310-00007

PMID:8856677

Abstract

Antisera were developed that specifically recognize orphanin FQ/nociceptin, the 17 amino acid peptide reported to be the endogenous ligand for the orphan opioid receptor. Immunocytochemical localizations in rat spinal cord demonstrated that orphanin FQ /nociceptin-immunoreactivity (-ir) was abundant in superficial dorsal horn, lateral spinal nucleus and the region dorsal to the central canal, areas that also exhibit prominent enkephalin-and dynorphin-ir. Orphanin FQ/nociceptin-ir was not affected by dorsal rhizotomy, indicating that in spinal cord the peptide is produced by central rather than primary afferent neurons. thus, the distribution of orphanin FQ/nociceptin-ir appeared in neuronal circuits that parallel those containing enkephalin- and dynorphin-ir, with only modest co-existence of these peptides.

摘要

已研制出特异性识别孤啡肽/痛敏肽的抗血清，该17氨基酸肽据报道是孤儿阿片受体的内源性配体。大鼠脊髓的免疫细胞化学定位表明，孤啡肽/痛敏肽免疫反应性（-ir）在浅表背角、外侧脊髓核以及中央管背侧区域丰富，这些区域也显示出明显的脑啡肽和强啡肽免疫反应性。孤啡肽/痛敏肽免疫反应性不受背根切断术的影响，表明在脊髓中该肽由中枢神经元而非初级传入神经元产生。因此，孤啡肽/痛敏肽免疫反应性的分布出现在与含有脑啡肽和强啡肽免疫反应性的神经元回路平行的神经元回路中，这些肽仅适度共存。