去势及雌激素处理后大鼠腹侧前列腺和Dunning R3327 PAP前列腺肿瘤中转化生长因子-β1的表达
Expression of transforming growth factor-beta 1 in rat ventral prostate and Dunning R3327 PAP prostate tumor after castration and estrogen treatment.
作者信息
Lindström P, Bergh A, Holm I, Damber J E
机构信息
Department of Urology, Umeå university, Sweden.
出版信息
Prostate. 1996 Oct;29(4):209-18. doi: 10.1002/(SICI)1097-0045(199610)29:4<209::AID-PROS2>3.0.CO;2-6.
BACKGROUND
In normal prostate, TGF-beta 1 is associated to castration induced apoptosis. Combined castration and estrogen treatment, but not castration alone, induces apoptosis in the Dunning R3327 PAP adenocarcinoma.
METHODS
TGF-beta 1 expression in rat ventral prostate (VP) and Dunning R3327 PAP tumor was studied after castration and estrogen treatment, using competitive RT-PCR, in situ hybridization and immunohistochemistry.
RESULTS
TGF-beta 1 mRNA level was 6 times higher in the tumor than in the VP. Combined castration and estrogen treatment increased TGF-beta 1 mRNA levels in the tumor from day 3, while castration did not. The TGF-beta 1 expression was located in the epithelial cells.
CONCLUSIONS
The Dunning R3327 PAP tumor contains high levels of TGF-beta 1, which are further increased by combined castration and estrogen treatment. However, since this increase is not apparent until day 3, TGF-beta 1 probably does not contribute to the known induction of apoptosis in the tumor at day 1 after combined castration and estrogen treatment.
背景
在正常前列腺中，转化生长因子β1（TGF-β1）与去势诱导的细胞凋亡相关。联合去势和雌激素治疗可诱导Dunning R3327 PAP腺癌发生细胞凋亡，而单独去势则不能。
方法
采用竞争性逆转录聚合酶链反应（RT-PCR）、原位杂交和免疫组织化学方法，研究去势和雌激素治疗后大鼠腹侧前列腺（VP）和Dunning R3327 PAP肿瘤中TGF-β1的表达。
结果
肿瘤中TGF-β1 mRNA水平比VP中的高6倍。联合去势和雌激素治疗从第3天起可增加肿瘤中TGF-β1 mRNA水平，而去势则无此作用。TGF-β1表达位于上皮细胞中。
结论
Dunning R3327 PAP肿瘤含有高水平的TGF-β1，联合去势和雌激素治疗可使其进一步升高。然而，由于这种升高直到第3天才明显，TGF-β1可能对联合去势和雌激素治疗后第1天肿瘤中已知的细胞凋亡诱导作用没有贡献。
Zotero 插件